This experiment was conducted to evaluation usage different levels of Choline Chloride Supplement (CCS) (0, 500mg/kg and 1000mg/kg) in the basal diet (corn and soybean meal) and effects on the Japanese quail liver and blood chemical parameters include cholesterol (HDL and LDL) and triglyceride contents. A total of 135 Japanese quail were randomly divided in to 3 experimental treatments with 3 replicates (15 birds per pen) and arranged in a completely randomized design. The experimental period lasted 6 weeks and during this period, the birds had free access to feed and water. Experimental diets consisted of: Basal diet without choline chloride supplement, basal diet with 500mg/kg choline chloride supplement and basal diet with 1000mg/kg choline chloride supplement. These diets were isonitrogenous and isoenergetic were given to Japanese quail throughout a 42-d growth period. Data was analyzed with one way ANOVA and means compared with Duncan test. Result for liver chemical analyses showed choline chloride supplement in levels of 500mg/kg and 1000mg/kg (T3 and T2 respectively) decrease the cholesterol and triglyceride contents numerically, and blood chemical analyses showed treatment of 3 and 2 (contain 1000mg/kg and 500mg/kg choline chloride supplement, respectively) decrease the cholesterol and triglyceride contents significantly (P<0.0001), too choline chloride supplement could increase significantly high density lipoprotein cholesterol (HDL) content in blood, and no significantly affected on blood low density lipoprotein cholesterol (LDL) content. Choline chloride supplement was decrease LDL content in blood numerically.
