This experiment was carried out to evaluation usage different levels of Canola Oil (CO) (0, 2 and 4%) and Choline Chloride Supplement (CCS) (0, 500 and 1000 mg/kg) in the basal diets (corn and soybean meal) and their effect on the Japanese quail’s liver chemical parameters include cholesterol and triglyceride contents. This trial was conducted in 3 × 3 factorial experiment with Completely Randomized Design (CRD) with 270 male quail. All diets were isoenergetic and isonitrogenous and balanced with NRC recommendation. Result shown that interaction effects of CO and CCS could affect Result for liver and blood chemical analyses show that interaction effects of canola oil and choline chloride supplement could affect liver and blood cholesterol and triglyceride contents. Canola oil and choline chloride supplement in 6 and 9 treatment (T6 = 2% CO + 1000 mg/kg CCS and T9 = 4% CO + 1000 mg/kg CCS) could significantly decrease the liver cholesterol and triglyceride contents respectively (p<0.0001). Result shown the levels of 1000 and 500 mg/kg CCS is highest effect on the liver cholesterol content respectively. Afterward of 6 and 9 treatment, 8 and 5 treatment (T8 = 4% CO + 500 mg/kg CCS and T5 = 2% CO + 500 mg/kg CCS), could significantly decrease the liver cholesterol and triglyceride contents (p<0.0001). Generally choline chloride in all diet treatment can decrease the levels of liver triglyceride and cholesterol content significantly (p<0.0001). Blood chemical analyses showed treatment of 9, 8 and 6 decrease the cholesterol and triglyceride contents significantly (P<0.0001), too choline chloride supplement could increase significantly high density lipoprotein cholesterol (HDL) content in blood, and no significantly affected on blood low density lipoprotein cholesterol (LDL) content. Choline chloride supplement was decrease LDL content in blood numerically.
