In order to consider the allelopathic effect of aqueous extracts of Artemisia annua on germination and early growth of Isabgol (Plantago ovate), an experiment was conducted using a completely randomized design with five treatments (control, 25%, 50%, 75% and 100% concentration of aqueous extracts) and four replications in the laboratory. Results analyzed by SAS software with means subjected to Duncan test at 5% probability level showed that effect of different concentrations of Artemisia annua extract was significant on germination percentage and rate, plumule and radicle lengths and fresh and dried weights of seedlings.