Annals of Biological Research
Abstract
Author(s): Meisam Mojarabi, Seyed Mohammad Hosseini Nasr, Hamid Jalilvand, Yahya Kooch
In order to conquest of ash seed dormancy, we used seeds of this species that were collected
from Haftkhal region, northern Iran, during 2005, 2007 and 2008 years by Forest Seed Center of
Khazar (FSCK). Seeds were sterilized with mercuric colorid 1% for 20 min, and using 70%
ethanol for 30 sec. Then half of seeds put in Gibberellic acid 500 mg/l density, the other half of
seeds put in sterile distilled water for 24 hours. Then each group of seeds cultured on MS and
enrich - MS medium with Ascorbic acid, Casein hydrolisat and yeast each 100 mg/l and 5 mg/l
activated charcoal. In general for each year seeds 6 treatments, for each treatment 5 replication
and in each replication 6 ash seeds have been cultured. Analysis of variance is showing that
storage period had an influence on germination index in %5 confidence limit. Also for plantlet
length, germination index and vigour after that period had a difference in %1 confidence limit.
In this study the most desirable period for seed storage was 1 year, so after this period the
germination index percentage (%14.815), plantlet length (2.766 mm) germination rate (2.601),
germination index (0.756) and vigour (2.676) had increased. Also we observed meaningful
difference on %1 confidence limit for effect of treatments for all those values, which in treatment
number 6 with using Giberlic acid, growth supplement and activated charcoal showed an
increasing in the germination percentage (%53.706), plantlet length (6.49 mm), germination rate
(7.584), germination index (4.235) and vigour (8.351). In analyses variance any meaningful
differences observed between seed storage and kind of treatments.
