Root anatomy of Lithocarpus urceolaris seedlings inoculated with Scleroderma spp. at different density of shading net was studied in greenhouse and in laboratory. L. urceolaris seedlings were inoculated with S. sinnamariense, S. columnare and S. citrinum at different of shading net (55 %, 65 % and 75 %) where the fungi were isolated from indigenous Fagaceae grown at Botanical Garden of Andalas University. The roots were studied for anatomical structures of mantle and Hartig net, percentage of ectomycorrhizal colonization and morphology of roots infected. The results showed that the roots of L. urceolaris could associate with all ectomycorrhizal fungi tested. The roots inoculated with S. columnare and S. citrinum under 65 % shades while S. sinnamariense in all condition shading density (55%, 65% and 75%) could give the best results with 60% colonization and catagorized as “good” colonization. L. urceolaris seedlings without shade, the colonization by the three ectomycorhizal of Scleroderma spp was around 30 %, and catagorized as “medium”. In uninoculated seedling, no formation of ectomycorrhiza was observed. The structure of root anatomy of seedlings inoculated with S. sinnamariense, S. columnare and S. citrinum showed that the seedling inoculated with S. columnare using 65% of shade, some of its mantles formed of three layers with the thickness of mantle 300 μm. Two layers of mantles were found under 55 % shade with inoculant S. sinnamariense and S. columnare with mantle thickness 200 μm and 75% shade, with inoculant S. sinnamariense mantle thickness was 150 μ and some formed one layer of mantle (single layer). Ectomycorrhiza formed had the same morpholocical characters, i.e. producing monopodial branches and the color of surface of seedling roots colonized by mycorrhiza was white because there was mysellia which covered roots.
