Der Pharmacia Lettre
Abstract
Author(s): Rujuta D. Patel and Dilip G. Maheshwari
The present manuscript describe simple, sensitive, rapid, accurate, precise and cost effective dual wavelength
spectrophotometric method for the simultaneous determination of Torsemide (TOR) and Amiloride HCl (AMI) in
combined tablet dosage form. The utility of dual wavelength data processing program is its ability to calculate
unknown concentration of components of interest in a mixture containing an interfering component. The principle
for dual wavelength method is �¢����the absorbance difference between two points on the mixture spectra is directly
proportional to the concentration of the component of interest. The method was based on determination of
Torsemide at the absorbance difference between 258.20 nm and 297.40nm and Amiloride HCl at the absorbance
difference between 276.80 nm and 300 nm. The linearity was obtained in the concentration range of 5-25 �Ž�¼g/ml for
Torsemide and 5-25 �Ž�¼g/ml for Amiloride HCl. The accuracy and precision of the method was determined and
validated statically. The method showed good reproducibility and recovery with % RSD less than 2. Method was
found to be rapid, specific, precise and accurate, can be successfully applied for the routine analysis of Torsemide
and Amiloride HCl in bulk, and combined dosage form without any interference by the excipients. The method was
validated according to ICH guidelines.
