Annals of Biological Research
Abstract
Author(s): Mahadevappa Paramesha, ChapeyilKumaran Ramesh, Venkatarangaiah Krishna and Sathyanarayana Raghavendra
The shoot tip, hypocotyls and cotyledons from the In vitro generated seedlings served as explant source for direct organogenesis in the present study. The influence on In vitro growth response and regeneration potentials of the shoot tip, hypocotyls, and cotyledons of safflower were evaluated on MS medium supplemented with different cytokines viz., TDZ/BAP/Kn and NAA (0.5-3.0 mg/l and 0.25-1.0 mg/l) evaluated. Among the different explants, the maximum mean shoot induction was recorded from shoot tip while cotyledons recorded minimum shoots induction. A protocol for large scale production of plantlets from shoot tip explant was standardized on MS medium supplemented with 1.5 mg/l TDZ and 0.5 NAA mg/l and 2.0mg/l BAP and 0.25 mg/l NAA. The In vitro derived microshoots from different explants were induced root initials on MS half strength medium supplemented with a range of 0.1 to 0.5mg/l NAA. The rooting response was maximum at 0.5 mg/l NAA supplemented medium. Complete rooting was observed within 45 days of culture. The rooted plantlets were subjected to an acclimatization process
