Hepatitis C belongs to the family of Flaviviridae and it causes the most serious viral hepatitis infections. Clinical problems ncirrhosis, chronic hepatitis, and hepatocellular carcinoma due Hepatitis C virus (HCV). The HCV genotype frequency in Pakistan is still missing, therefore it’s very important to investigate the HCV genotypes in infected patients. Molecular techniques play a very important role in the diagnosis of viral infection. In this study, we detect the different genotypes of HCV by using Polymerase Chain Reaction with the help of specific primers., firstly the samples were collected from different areas of Faisalabad and DNA extraction was performed. Then PCR was performed by using specific primers. After that, the PCR-positive samples were used for genotyping by using a methodology with minor changes. This study shows the different genotypes of 2a, 1b, and 3a. The most prevalent genotype was 3a, 1b, and 2a with 60%, 20%, and 10% respectively. remaining samples were untypeable and investigate for further analysis. For further analysis and epidemiology, sequencing of these genotypes was useful in diagnosis.
