Annals of Biological Research
Abstract
Author(s): Sajidur Rahman Akash,
Md Imran Hossain and
Md Sarafat Ali*
Dengue is one of the major mosquito-borne diseases that still threaten humans and kill countless people. It is caused by a
positive-stranded RNA virusthat included the family of Flaviviridae. Dengue fever is an exquisite feverish viral disease
dispatched by Aedes mosquitoes? sting, posing any one of the four dengue viral serotypes. This virus transmits through
a vertical transmission way using a full unique system. Unfortunately, there is no still effective developed vaccine to
eradicate this disease. Computational methods were used in this work to build and we suggest a multi-epitope vaccination
for the dengue virus in Asia. Epitope conservation was taken into account because Dengue virus is an RNA virus, and all
selected epitopes were 100% conserved. Antigenicity of the final multi-epitope vaccine component was 0.7055. Disulfide
engineering was conducted at an area of high mobility to improve vaccine protein stability. In addition, codon adaptation
and in silico cloning was used to guarantee that the planned subunit vaccine in E. coli was expressed at a greater level.
Lastly, a molecular docking and simulation analysis was carried out for the vaccination protein and the TLR-4 receptor in
order to assess the binding free energy and stability of the combination for this reason, the suggested in silico vaccine has
to be tested forsafety and immunogenicity in orderto guarantee an active immunity against the Dengue virus
