Vascular endothelial growth factor (VEGF) is an endothelial cell-specific mitogen that increases peripheral oxygen delivery by stimulating angiogenesis. VEGF is known to be expressed in several fetus tissues and placenta. VEGF is initially expressed in the yolk sac and in embryonic sites of vessels formation to support the assembly of a cardiovascular system. It is instructive not only for cardiovascular development, but also for the development of organ systems. The amnion forming the amniotic cavity, providing an aqueous environment for the embryo. A wide range of proteins including growth factors and cytokines has been identified in human amniotic fluid (AF). In this study, total protein concentration (TPC) and VEGF level in the amniotic fluid samples from chick embryos were measured using dye-based protein assay and enzyme linked immunosorbent assay (ELISA). TPC decreased from days E6 to E10 and increased from E11 to E15. However, the amount of AF VEGF was increased from day E6 to E11 and thereafter the levels decreased from days E11 to E15. Since VEGF is important in the development of the organ systems, changes in the TPC and VEGF levels in AF during chick embryonic development may be correlated with organ development. We have also concluded that VEGF is a constant component of chick AF.
