Standardization of an Ayurvedic polyherbal formulation is important for efficacy and quality control aspect of Ayurvedic polyherbal medicines. The modern methods can be used for standardization of these formulations. The qualitative and quantitative study of herbal drug can be accomplished by simple, rapid and cost effective UV Absorption Specrophotometric method. In the present study the Ayurvedic polyherbal formulation used as a brain tonic, was analyzed by a UV specrtophotometric method using shankhpushpin (Evolvoulous alsinoide) and asiaticoside (Centella asiatica) as marker compounds. The validation parameter as per ICH guidelines such as linearity, Precision, accuracy, limit of detection and limit of quantitation was studied. The linearity was observed in the range of 5-30μg/ml for both the markers. Limit of detection and quantitation was observed in nanograms showing high sensitivity of the developed method. The percent recovered amount of was 97.28 % Shankhpushpin and 95.69% Asiaticoside. The method was robustness and ruggedness for analysis of polyherbal formulation. The polyherbal Ayurvedic formulation can be analyzed successfully by UV Absorption Spectrophotometric method
