Annals of Biological Research
Abstract
Author(s): A. K. M. Mohiuddin, Nilufer Hye Karim and Shahanaz Sultana
An intensive study was carried out to achieve green plant regeneration as well as to develop doubled-haploid lines (DHLs) from anthers of advanced breeding line, BR802-78-2-1-1. Response of cultured anthers to callus induction found in KA (no salt) by 2.9% and in KC (5.0 g/l NaCl) by 8.7%. Percent plant regeneration as well as production of green plants increased under salt stress. Data based on seed morphological characteristics of well developed DHLs were subjected to cluster analysis for grouping into types; designated as AC (anther culture) and numbered in ascending order of transferring to earthen pots. At least eight types of variants with a wide range of differences were conceivable including a type similar to the parent, BR802-78-2-1-1. Comparative analysis showed that all the variants had plant height lower than the parent. In respect of effective tillers, an important indicator of grain yield, AC156 and AC192, produced 100% effective tillers and only 4 and 3% grain sterility, respectively. The spikelet number per panicle was 306 and 294 with long-bold and long-slender grain in AC156 and AC192, respectively. The physical characteristics and performance of the AC lines revealed the potentiality of the doubled-haploids in fixing segregating populations. The H1 types will be grown for confirmation of homozygosity in the transplanted aman and boro seasons and further yield trails leading to variety development
