A novel reverse phase liquid chromatographic method was developed and validated for simultaneous estimation of antiseptic drug, boric acid and antifungal drug, chlorphenesin in its dosage form i.e. prickly heat powder. Refractive index detector was used for detection of eluted components. It is most suitable for quantification of boric acid because boric acid could not be detected by UV detector as it does not contain chromophore group. The reverse phase HPLC analysis was carried out on isocratic system. The column used was Inertsil C8-4 (250mm x 4.6mm, 3μm) with operating temperature at 34° C. The mobile phase consisted of water: acetonitrile in proportion 70: 30 % (v/v). The flow rate was maintained at 0.5 ml / min with detector temperature set at 34°c and sensitivity at 512. The method was validated as per ICH guidelines for system suitability, linearity, accuracy and precision. The linear ranges were 1753.2 – 5611.9 μg/ml for boric acid and 503.8 – 1612.1 μg/ml for chlorphenesin. The accuracy and precision were found to be well within the acceptable limit. The method was successfully applied for simultaneous determination of boric acid and chlorphenesin in prickly heat powder with good recoveries.
