Simultaneous estimation of Tramadol HCl and Quercetin (Dihydrate) using a specific, rapid and simple UV spectrophotometric method with good sensitivity in niosome formulation has been developed. From the optical characteristics of the proposed methods, it was found that the λ max of Tramadol HCl and Quercetin(Dihydrate) was found to 271 nm and 372 nm respectively. Tramadol HCl and Quercetin (Dihydrate) obey linearity within the concentration range of 0 – 20 μg/ml and 2-20 μg/ml. Recovery study was performed to confirm the accuracy of the method. According to ICH guidelines Method development and validation has been adopted for routine estimation of Tramadol HCL and Quercetin (Dihydrate) niosome formulation.
