Reversed phase liquid chromatographic (RP-HPLC) method has been developed and subsequently validated for the determination of Glibenclamide in pure and formulations. Standard stock solution (1.0 mg.mL-1) of Glibenclamide was prepared by transferring accurately weighed 50.0 mg of Glibenclamide and dissolving in a 50 mL volumetric flask containing 10 mL of methanol. This solution was sonicated for 20 min to achieve complete dissolution and made up to the mark with mobile phase. From the standard stock solution different concentrations of working standard solutions of Glibenclamide were prepared with the same mobile phase ranging from 2.0 -10.0 μg.mL-1. The linearity for HPLC method was determined at six concentration levels ranging from 2.0 -10.0 μg.mL-1 for Glibenclamide. The developed method offers several advantages in terms of simplicity in mobile phase, mode of elution, easy sample preparation steps and comparative short run time which makes the method specific and reliable for its intended use in routine analysis determination of Glibenclamide in tablet dosage forms.
