A new, simple, rapid, selective, precise and accurate isocratic reverse phase high performance liquid Chromatography assay method has been developed for estimation of Lisinopril in tablet formulations. The separation was achieved by using column Hypersil BDS C18, 250 × 4.6 mm, 5 μm, in mobile phase Phosphate buffer pH 5.0±0.05 and Acetonitrile in the ratio of 96:4 v/v. The flow rate was 2.0 mL.min-1 and the separated Lisinopril was detected using UV detector at the wavelength of 210 nm. The retention time of Lisinopril, was noted to be 3.68 min respectively, indicative of rather shorter analysis time. The method was validated as per ICH guidelines. The proposed method was found to be accurate, reproducible, and consistent.
