There are many analytical methods available for estimation of Albendazole and Levamisole HCl separately in pharmaceutical preparations. However, no specific RP-HPLC method is available for simultaneous estimation of Albendazole and Levamisole HCl in pharmaceutical dosage form. A simple, rapid and accurate RP-HPLC method was developed and validated as per ICH Q2 (R1) guidelines for the estimation of Albendazole and Levamisole HCl in pharmaceutical dosage form. Method development was carried out on phenomenex C18 isocratic column, (250 mm × 4.6 mm i.d., particle size 5 μm, maintained at ambient temperature) LC-20 AD Prominence Liquid chromatograph (shimadzu, Japan) attached with SPD-20A/20AV Prominence UV/Vis detector. The mobile phase was a mixture of 0.02 M potassium dihydrogen orthophosphate buffer and Methanol in ratio 40:60 v/v, the flow rate was set at 1.0 ml/min and UV detection at 219 nm. In the linearity study, linearity of Albendazole was observed from 28-52μg/ml with correlation coefficient of 0.999and linearity of Levamisole HCl was observed from 10.5-19.5μg/ml with correlation coefficient of 0.999. The developed RP-HPLC method for the quantification of Albendazole and Levamisole HCl has various advantages like good peak symmetry, less retention time and phenomenal linearity, highly sensitive, accurate, precise, simple and robust.The proposed method can be used for the routine analysis of Albendazole and Levamisole HCl in pharmaceutical dosage forms for routine application in quality control laboratories without interference of excipients.
