A novel and very rapid, sensitive, reverse phase Ultra Performance Liquid Chromatography (RP-UPLC) technique was developed for the quantitative estimation of naproxen in dosage form, as there is no official monograph & no analytical method by UPLC. The developed UPLC has several advantages over conventional HPLC with respect to speed, solvent consumption, resolution and cost of analysis.It was resolved by using a mobile phase of Potassium dihydrogen phosphate: methanol in the ratio 30:70 v/v at a flow rate of 0.8 mL/min. using UV - Visible detector at the wavelength of 287 nm for quantification. Efficient separation was achieved for naproxen on Waters Acquity HSS T-3 C18 column (100 × 2.1 mm, 1.8μm). The retention time of naproxen was 2.852 min. The calibration graphs were linear and the method showed excellent recovery for tablet dosage form. The developed method was validated according to the International Conference on Harmonization (ICH) guidelines with respect to linearity, accuracy, precision, specificity and robustness.
