For the assay of Valacyclovir hydrochloride five new spectrophotometric methods have been developed in pharmaceutical dosage forms. The first method was developed in Sodium Acetate (Method A) showing absorption maxima at 251 nm, the second method Phosphate buffer pH 5.0 (Method B) was used (λmax 251 nm), the third method phosphate buffer pH 7 (Method C) was used (λmax 252 nm), the fourth method borate buffer pH 9.0 (Method D) was used (λmax 253 nm) and the fifth method 0.1N NaOH (Method E) was used (λmax 265 nm). Linearity was observed over the concentration range 1-80 μg/mL for all the methods A, B, C, D and E respectively with regression equations 0.032x + 0.0144, 0.0324x + 0.0044, 0.0322x + 0.0118, 0.0304x + 0.0013 and 0.0278x + 0.0063 for method A, B, C, D and E respectively. The proposed spectrophotometric methods were validated and can be applied for the determination of Valacyclovir Hydrochloride in pharmaceutical formulations.
