A simple isocratic, rapid and sensitive high performance liquid chromatographic method has been developed for quantitative determination of Trazodone hydrochloride and its three process related impurities. The method has been validated for determination of related substance in Trazodone hydrochloride using C8 ODS (150X4.6mm) column by keeping the flow rate of 1ml/min and having sensitivity of 1.The elution is carried out by using mobile phase of 300ml acetonitrile,50ml THF,400ml water and 250 ml methanol and pH was adjusted to 11 by TEA. The detection is carried out at 255nm with injection volume of 10 microlitre. Specificity, system suitability, linearity, precision, ruggedness, robustness has been carried out for Trazodone hydrochloride. Limit of quantification and limit of detection has been carried out for the impurities of Trazodone hydrochloride. Forced degradation study of Trazodone hydrochloride been carried out .Impurity profiling is also been carried out.
