Der Pharmacia Lettre
Abstract
Author(s): A. B. Devkhile, K. A. Shaikh
In this perspective, we present a reverse phase high performance liquid chromatographic
method for simultaneous determination of piroxicam and paracetamol in commercial
pharmaceutical dosage forms. The resulting contents of tablets were baseline resolved on a
reverse phase Zorbax SB C18, (250 x 4.6 mm, 5 mm) analytical column. Mobile phase contains
methanol, acetonitrile and 50 mM sodium dihydrogen orthophosphate (27:16:57 v/v) at a flow
rate of 1 mL/min. UV detector was set at a wavelength of 254 nm. The resolution between
paracetamol and piroxicam was less than five. Developed method was extensively proved to be
robust for the titled drugs. This method was shown to be linear, correlation coefficient of
paracetamol and piroxicam was 0.9986 and 0.9990 respectively. The percent recovery for
paracetamol and piroxicam ranged between 100.5-101.3 and 100.6-100.8 respectively. The
percent relative standard deviation for six replicates was less than 2 .The limit of detection and
limit of quantification for paracetamol was 140 ng / mL and 400 ng / mL for piroxicam 29 ng /
mL and 70 ng / mL . Sample concentrations were measured on weight basis to avoid internal
standard. Proposed method is suitable for quantitative determination of the titled drugs in their
commercial samples of tablet formulation with respect to assay.
