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Development and validation of HPLC method for estimation of lindane in its formulation. | Abstract
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Abstract

Development and validation of HPLC method for estimation of lindane in its formulation.

Author(s): Ravi Challa and N. V. S. Naidu

A simple, economic, selective, precise and accurate High Performance liquid Chromatographic method used for the analysis of Lindane in its Formulations. Formulations was developed and validated in the present study. The mobile phase consists of mixed acetonitrile and water in the proportion 50:50 respectively. And this was found to give a sharp peak of Lindane at a retention time of 7.943 min. HPLC analysis of Lindane was carried out at a wavelength of 254 nm, With a flow rate of 2.08 ml min-1 linear regression analysis data for the calibration curve showed a good linear relationship with regression coefficient 0.999 in the concentration range of 50 ppm to 150 ppm. The linear regression equation was Y=1825×+484.8 the developed method was employed with a high degree of precision and accuracy for the analysis of Lindane. The method was validated for accuracy, precision, robustness, detection and quantification limits as for ICH guidelines. The wide linearity range, accuracy, sensitivity, short retention time and composition of the mobile phase indicate that this method is better for the quantification of Lindane