Der Pharmacia Lettre
Abstract
Author(s): Anandakumar Karunakaran, Kannan Kamarajan, Vetrichelvan Thangarasu
This paper describes validated First Derivative Spectrophotometric method for the simultaneous
estimation of Lamivudine and Tenofovir disoproxil fumerate in pure and in formulation. The
solutions of standard and sample were prepared in distilled water. Quantitative determination of
the drugs was performed at 287 nm and at 249 nm (N = 1; �Ž�» = 1) for Lamivudine and Tenofovir
disoproxil fumerate, respectively. Proposed method was evaluated for the different validation
parameters. The specificity test showed that there was no interference from excipients commonly
found in the commercial pharmaceutical formulations at the analytical wavelengths of LAM and
TDF. Quantification was achieved over the concentration range of 5 Ã?¢Ã?â?¬Ã?â?? 30 Ã?ŽÃ?¼g/ ml for Lamivudine
and 10 Ã?¢Ã?â?¬Ã?â?? 60 Ã?ŽÃ?¼g/ ml for Tenofovir disoproxil fumerate. The mean recovery was 100.27 Ã?â??Ã?± 1.2511
and 100.70 Ã?â??Ã?± 1.0604 % for LAM and TDF, respectively. This method is simple, precise, and
sensitive and applicable for the simultaneous determination of LAM and TDF in pure powder
and formulation.
