There is rare discussion available on quantitative separation of optical isomers of Dolutegravir, Dolutegravir is most recent FDA approved antiretroviral drug. The author has described simple, sensitive, precise, and specific RP-HPLC method for the separation of (R,R)-diastereomer, (S,S)-diastereomer and (S,R) enantiomer of Dolutegravir from its Process related and degradant impurities using Chiralpak IF-3, HPLC column. The method is validated as per ICH Q2(R1) guideline and can be used in routine analysis.
