A new precise, accurate, rapid method has been developed for the simultaneous estimation of Bromofenac and moxifloxacin in pharmaceutical dosage form by RP-HPLC. The method was optimised with Mobile phase Phosphate buffer: Acetonitrile (40:60), Column Hypersil BDS,C18 flow rate 0.1μml, detection wave length at 275 nm .Retention time for bromofenac & moxifloxacin was found to be 2.3min & 3.6min with the above conditions. System suitability parameters were studied by injecting the standard five times and results were well under the acceptance criteria. Linearity studies were carried out between 22.5μg/ml-135lμg/ml and 125μg/ml-750μg/ml levels of Bromofenac and moxifloxacin , R2 value was found to be 0.999. Accuracy was determined at three different levels 50%, 100%, 150% and %mean recovery studies were found to be 99.83, 100.9, 99.82 and 99.70, 99.66, 100.39 for Bromofenac and moxifloxacin respectively.The method is precise as the %RSD values of peak areas for Moxifloxacin and Bromofenac were found to be 0.39 and 0.40 respectively which are well within the acceptance criteria limit (RSD ≤ 2).The method was subjected to degradation studies through acid, base, photo stability degradations. In all the cases the % degradation is very less, the purity angle was found to be less than threshold and there are no co-eluting peaks near the Rt of the drugs. So we assume that the method is specific and stable one .Hence we conclude that the method is simple, specific, rapid, linear, accurate, precise, robust, stable and economical. Therefore, this method can be used for the estimation of bromofenac & moxifloxacin in Pharmaceutical dosage form for routine analysis purpose.
