Two simple, sensitive, highly accurate UV-Visible spectrophotometric methods have been developed for the determination of Cytarabine in bulk and pharmaceutical dosage forms. Method A is the UV Spectrophotometric method by using distilled water exhibiting λ max at 277.5 nm. Method B is based on the Imine complex with the PDAB reagent to form a colored chromogens exhibiting λ max at 455 nm. Beer’s law obeyed in the concentration range for Method A and B are 2-12 μg/ml and 10-50 μg/ml respectively. The slope, intercept, correlation coefficient was calculated. The proposed methods have been successfully used for analysis of the drug in pure and its tablet dosage forms. The results of percentage recovery show that the method was not affected by the presence of common excipients. The percentage assay of Cytarabine was 100.42. The method was validated as per ICH guidelines and can be used for the determination of Cytarabine in bulk and pharmaceutical formulation.