In order to gain and sustain a competitive advantage in the global economy, today’s organizations need to effectively mobilize their knowledge resources. Knowledge management is the organizational optimization of knowledge to achieve enhanced performance through the use of various methods and techniques. the purpose of this study was to determine the relationship between organizational structure and knowledge management in employees of sport organization of Iran Islamic Republic. The method of this research was descriptive-survey and correlation. First data gathered by field method via censes of staff managers of physical education organization by two questionnaires. The content validity of these questionnaires was confirmed by officers of management faculty of university of Tehran and their reliabilities were obtained by Krunbakh Alpha again (KM=0/89 and organizational structure=0/86). Finally 107 questionnaires were returned and analyzed Results were optioned by SPSS in tow levels of descriptive (internal tendency, variability) and inferential (spearman and Pearson correlation) Statistics. The significant relationship was showed between formalization with knowledge creation and transfer(p= 0/009) (p= 0/008) and high level of formalization with down levels of creation and transfer of knowledge were correlated (r=-0/27)(r=-0/365).The relationships between centralization and creation and transfer of knowledge were significant (p=0/017)(p=0/005) and high level of centralization with down levels of creation and transfer of knowledge were correlated (r=-0/401)(r=-0/485). There was significant relationship between complexity and creation and transfer of knowledge (p=0/041) (p=0/023) that high level of complexity correlated with high level of creation and knowledge transfer(r=-0/145)(r=- 0/185).The relationship between creation and transfer of knowledge was significant (p=0/002) which high levels of those were correlated (r= 0/652). With corrective of organizational structure can provide field for application of knowledge management.