Seeds of plants belonging to Solanaceae family are well known for their alkaloidal secondary metabolites. But there exists some non-alkaloidal secondary metabolites which are less explored. The objective of the present study was to determine the amount of bioactive non-alkaloidal secondary metabolites which is called Rutin in the seeds of the medicinal plant, Hyoscyamusniger, which is commonly known as henbane .In this study ,the amount of rutin content was quantitatively determined in H.niger seeds which was collected from three different regions of Iran in july, using high performance liquid chromatography (HPLC) . The amount of rutin on dry matter was 25/76(mg/g) for Kermanshah ,24/71(mg/g) for Black grove kandovan and 11/22(mg/g) for Karaj.Rutin content in seeds provided more importance tohenbane as a medicinal plant.
