Rutin is a bioflavonoid with the strong antioxidant properties which has been found to occur abundantly in some plant .Also it is one of the most abundant flavonoid in Capparis spinosa. The present study was to determine rutin content in all parts of caper plant at the vegetative stage and results of three analytical methods (HPLC and two spectrophotometric) was evaluated.C. spinosa was collected from Tafresh/Iran inJun. Plants separated into leaf, stem and root which were dried separately, and subsequently assayed for rutin content. Among different organs, leaves had higher rutin contents. Comparing three analytical methods, HPLC and ALCL3 are the best method for rutin measurement in C.spinosa.
