The determination of profenofos pesticide residue in tomato (Solanum lycopersicum L.) had been investigated by using Gas Chromatography technique with Flame Photometric Detector (FPD). The tomato was collected from Koto Baru, Tanah Datar, West Sumatera, Indonesia. The samples were divided into three groups: unwashed tomato, washed with tap water and washed with detergent. Samples were extracted once by ultrasonication for 10 minutes with 100 mL ethyl acetate. Results showed that the tomato contained profenofos pesticide residue. The unwashed tomato, washed with tap water and washed with detergent contains 0.159±0.0079; 0.070±0.0009 and 0.067±0.0016 ppm respectively. This data was obtained from the measurement as much as three times on each sample. The profenofos residue levels do not exceed the Maximum Residue Limit (MRL) that established by Indonesian National Standard (in ppm). Statistical tests with one-way ANOVA (SPSS 20.0) showed there was decrement in the levels of profenofos pesticide residues significantly between unwashed tomatoes, tomatoes washed with tap water and tomatoes washed with detergent (P <0.05).