Rapid and accurate reverse phase high performance liquid chromatography method is described for determination of ofloxacin from the bulk drug and pharmaceutical dosage form. It was observed that Polaris C18 (15 x 4.6 mm i.d.) with 5 μ particle size column showed most favorable chromatographic pattern over the other columns. The mobile phase consisted of buffer and acetonitrile (80:20 % v/v). The buffer was mixtures of 0.01 M ammonium acetate adjusted the pH 3 with ortho-phosphoric acid. The detection was carried out at wavelength 294 nm. The method was validated for system suitability, linearity, accuracy, precision, robustness and stability of sample solution with the linear range 10-30 μg/ ml. The method has been successfully used to assay of pharmaceutical dosage form i.e. tablets with good recoveries.
