Rapid and accurate reverse phase high performance liquid chromatography method is described for determination of fexofenadine hydrochloride from the pharmaceutical dosage form. It was observed that Polaris C18 (15 x 4.6 mm i.d.) with 5 μ particle size column showed most favorable chromatographic pattern over the other columns. The mobile phase consisted of buffer and acetonitrile (65:35 % v/v). The detection was carried out at wavelength 220 nm. The method was validated for system suitability, linearity, accuracy, precision, robustness and stability of sample solution with the linear range 5-15 μg/ ml. The method has been successfully used to assay of pharmaceutical dosage form i.e. tablets with good recoveries.
