Calreticulin (CRT) protein has multifaceted role in apoptosis. Since the molecular mechanism that express and perform apoptotic cell death are coming into focus, we attempt to isolate the human calreticulin gene from blood, cDNA synthesized from the CRT was cloned to mammalian expression vector pcDNA to construct a recombinant CRT plasmid. Transfection of the MCF -7 cell line pcDNA3-CRT plasmid leads to cell death by promoting apoptosis. The transformed cells exhibited nuclear margination, membrane blebbing and chromatin condensation that are typical for cells death by apoptosis. The clonogenic survival assay of transformed MCF-7 cells revealed that CRT overexpression reduced MCF-7 cell proliferation and increased cell death.
