A simple and reliable Gas chromatography-Mass spectrometer method was developed for the
determination of Benzoic acid in fruit juice. Chloroform was used for extraction solvent and coextractive
was removed by ether and 0.5M NaOH. The method was validated by determining
parameters such as, specificity, linearity, limit of detection, limit of quantitation, precision,
recovery and robustness. The method was found to be specific against fruit matrix interferences.
Linearity was evaluated over the concentration ranges of 0.1 μg/ml to 10 μg/ml and correlation
coefficient was more than 0.999. Both the inter day and intra day precision of the system and
method were determined. Recovery data obtained by fortifying three matrices at 1.0 μg/g and 2.0
μg/g with ranged between 98 to 105 % and the relative standard deviation (RSD) was obtained
below 5%. Limit of detection and quantitation of benzoic acid were 0.05 and 0.1 μg/g.
