Phusion blood direct Polymerase Chain Reaction (PCR) Kit was used to detect P. falciparum adenosine triphosphatase-6 (PfATPase-6) and P. falciparum translationally controlled tumor suppressor protein (PfTCTP) genes of the malaria parasite for artemisinins in 20 treated patients with Artemisinin Combination Therapy (ACT). Amplification was observed in at least 1 gene locus in 80% of blood sample assayed for PCR. The gene coding for the enzyme targeted by Artemisinin which is a drug used in the treatment of Malaria was detected in the blood of treated patients by Polymerase Chain Reaction. Amplification was observed in at least 1 gene locus in 80% of blood sample assayed. Successful amplification in patients in whom there was P. falciparum parasitemia after full treatment with ACT was detected in the gene loci PfATPase-6,1 (35%), PfATPase-6,2 (15%); and PfATPase-6,3 (60%). No amplicons were detected in PfATPase-6,4 and PfTCTP genes.
