A 65KDa acetylcholinesterase enzyme was purified from hypocotyls of P. vulgaris. The purification was done with the help of Sephadex G-100 and the ion exchange chromatography on DEAE- Sephacel column. The active fractions were pooled and subjected to SDS PAGE for the confirmation of molecular weight. The purified enzyme was immobilized onto PVC strip for colorimetric detection. Immobilization was confirmed by SEM and FTIR. The effect of pH, temperature, time of incubation and effect of substrate concentration were also studied for both free and immobilized enzyme. The PVC enzymatic strip was also evaluated. The developed method can be exploited for the detection of OP compounds which are responsible for causing environmental pollution.
