Docking studies of twenty novel 5-nitro benzimidazoles was done with the help of VLife MDS3.5software using GRIP batch docking method by taking AT-2 receptor model with bovine rhodopsin crystal structure as a target. Based on the docking scores of the designedmolecules which ranged from -14.39 to -36.16 , the 5-nitro benzimidazole derivatives were synthesized by refluxing 4-nitro-1,2-phenylenedimaine in dimethoxyethane with various substituted aromatic aldehydes in the presence of sodium metabisulphite as oxiding agent. The test compounds were characterized by TLC, melting point, UV, IR, 1H NMR and mass spectroscopy.All the compounds were evaluated for ex-vivovasorelaxant activity in rat aorta rings pre-contracted with phenylepherine.Compounds BDZ3, BDZ6, BDZ12, BDZ18 and BDZ20 showed good vasorelaxant activity (EC50<30 μM).
