Rheumatoid arthritis is one of the most serious non-fatal conditions with significant prevalence around the globe. Objective: The objective of present work was to design and characterize Moringa oleifera seed oil impregnated anti-inflammatory topical micro-dispersion. The ternary phase diagrams for microemulsion regions were constructed using Moringa oleifera seed oil as oil phase, Tween 80 and Span 80 as surfactants. The microemulsion was characterized for its percentage transmittance, refractive index, pH, globule size, zeta potential, viscosity, invitro drug release, skin irritancy and anti-inflammatory activity. The batch F3 with 25.30% of oil, 59.02 % of Smix and 15.68% of water; was found to be more stable over other batches. The percentage transmittance and refractive index (RI) of formulation was found to be 99.60 and 1.469 respectively; whereas pH and average globule size was 7.1 and 167nm respectively. zeta potential and viscosity of optimized microemulsion was found to be -35.8 and 88.7 cps respectively. The permeability of Moringa oleifera seed oil microemulsion was 76.86% as compared to 54.06% of pure oil. The prepared microemulsion was found to be non-irritant and showed significant (p<0.05) antiinflammatory effect. The Moringa oleifera seed oil can be used to develop stable micro-dispersion system with better skin permeation and promising anti-inflammatory activity without any skin irritation.
