The aim of present work was to develop a simple, specific, accurate and precise validated HPTLC method for quantification of Lansoprazole and Aspirin in fixed dose combination. The chromatograms were developed using a mobile phase of Chloroform: Methanol: Glacial acetic acid (9:0.4:0.02,v/v/v) on pre-coated plate of silica gel GF254 aluminum TLC plate and quantified by Densitometric absorbance mode at 284nm. The Retardation factor (Rf) values of Lansoprazole and Aspirin were found to be 0.52 ± 0.06 and 0.24 ± 0.0047 respectively. The linearity of the method was found to be within the concentration range of 5- 50 μg/ spot and 32.5 – 325 μg/spot for Lansoprazole and Aspirin respectively. The lower limits of detection and quantification were 0.135 μg/ spot and 0.4091 μg/ spot for Lansoprazole and 0.527 μg/ spot and 1.597 μg/ spot for Aspirin. The method was validated for precision, specificity and recovery as per ICH guideline. %RSD values were found to be less than 2 and % recovery was between 99-102 %. The proposed validated HPTLC method was simple and rapid. The mean percentage assay values reveal that this method could be successfully utilized to analyze Lansoprazole and Aspirin in prepared laboratory mixture accurately without any interference of formulation additives.
