The development of in-vitro cytotoxicity assays has been driven by the need to rapidly evaluate the potential toxicity of large numbers of compounds. In the present study the anticancer study has been performed to the synthesized compounds (1-10) by determination of total cell protein content through sulphoradamine B (SRB) assay on HEp-2 (Human larynx cancer cell line). Compounds 1, 2, 9 and 10 showed minimum cytotoxic concentration (CTC50) at which 50 percent of cancer cell populations were inhibited and are comparable to the standard drug 5- Fluorouracil. Potent compounds of the series were selected for enzyme estimation to show any hepatic toxicity. All enzymes estimated were compared to the control. SGPT, SGOT values of compound 9 and alkaline phosphatase of compounds 9 and 10 were found to be moderately significant (p < 0.01).
