Cubosomes are liquid-crystalline nanoparticles. With two nonintersecting water channels, cubosomes posses plenty of advantages like enhanced stability, biocompability and presence of both hydrophilic and hydrophobic regions. According to that, cubosomes emerge as a great, potential systems for biomedical applications such as bioimaging or drug delivery. One of the main issue of investigations is determination of cytotoxicity in order to define the lethal concentration. In pursuance of defining the cytotoxicity, WST-1 and InCell assays on both MSU and HeLa cells have been conducted revealing modest differences between cell lines.