Parasitic diseases including leishmaniasis, malaria, and trypanosomiasis continue to pose a significant threat to human health around the world. While the genomes of Leishmania, Plasmodium, Trypanosoma, and Anopheles were sequenced over a decade ago, genome-editing techniques have hindered the research of gene function. As a result, the initial research in eukaryotes employing CRISPR/Cas9 gene editing opened up fascinating new prospects in parasitology.