Sugar beet, cultivated Beta vulgaris, is a plant whose root contains a high concentration of sucrose. It is grown commercially for sugar production. The root of the beet contains 75% water, about 20% sugar and 5% pulp. By considering this in the present study, Sugar beet roots were used to produce ethanol using Saccharomyces cerevisiae. After fermentation at 30O c for 48 hours ethanol was purified and estimated by modified dichromate method. For estimation 10ml of Acid dichromate solution was taken in BOD bottle. The ethanol sample from fermented broth was kept in hanging position in 2ml eppendroff vial for 24 hours. The ethanol from sample was converted in to ethanolic acid by dichromate solution. Remaining unused dichromate solution was then estimated with thiosulphate solution to determine ethanol percentage. Results indicated that this method is 99 % effective in estimation of ethanol and can be used as one of the best method for ethanol production.
