The carob moth, Ectomyelois ceratoniae Zeller, is a worldwide pest of nuts and fruits. This species is the most destructive pest of pomegranate in Iran. In this research, volatile components produced by carob moth were extracted by using Solid-phase microextraction (SPME) to compare the efficiency of different extraction techniques by SPME. SPME was conducted through two different methods: 1) extraction from headspace, 2) extraction through the contact of fiber with papillae anales of females or valva of males. Chemical analyses of extractions were conducted by Mass Spectrometry-Gas Chromatography (GC-MS). Results showed that more female specific components (3 components) were detected by extraction through the contact of fiber with papillae anales of females than the other metods of extraction by SPME. The results evidenced the role of extraction method on detection of female specific volatile components of carob moth and extraction through the contact of fiber with papillae anales of females could be optimized method for extraction of the female specific volatile components of carob moth.
