The objective of this study was conducted to evaluate the effects of Cuminum cyminum and Probiotic on performance and serum composition of broiler chickens. A total of 400 one day- old male Ross-308 broiler chicks were allocated into 4 dietary treatments and 5 repetitions with 20 chickens in each group, including P1) basal diet with no supplement as control group P2) basal diet containing 1% probiotic(L. acidophilus and L. casei). P3) received 2% Cuminum cyminum P4) Basal diet with 1% probiotic and 2% probiotic. The results showed that the best result of the live weight and FCR of the treated groups was in P4 (P<0.05), but the highest feed intake was in group 3. As compared to the control group with the other groups observably to give improve performance in all of the experimental (P<0.05). The highest percent of liver and breast was observed in experimental group 4 and the highest percent of gizzard and lowest percent of abdominal fat were in experimental groups 2. According to the results, total cholesterol (Chol), triglyceride (TG), HDL, LDL and Glucose were measured in blood samples of day 42. The amount of total Cholesterol, triglyceride (TG), LDL and HDL in the serum showed significant differences, but glucose was not significantly different among groups.