Swimming pools being some of the popular sport centers are able to potentially receive some pollutants such as nasal secretions, saliva and urine and endanger the health of swimmers. The aim of this study was to determine the chemical, biological and physical quality of swimming pools in Maragheh city. This research was a cross-sectional study. Totally, 120 water samples were collected from five public swimming pools in Maragheh, Iran. Microbial and physicochemical conditions were examined according to the standard method. The average of residual chlorine and pH in pools was 0.93±0.51 mg/L and 7.3±0.69, respectively. The maximum and minimum of pH were 8.1 and 6.95 and for residual chlorine were 2.06 and 0.24 mg/L respectively. Out of 120 water samples examined, 90 (75%) conformed to the microbiological standards specified, and 30 (25%) exceeded at least one of the indicated limits. The positive samples contained at least one or several types of bacteria. Results indicated that S. aureus had the highest percentage frequencies while the least was exhibited by E. coli. It is recommended that health authorities should pay more attention to cleaning and disinfecting, to prevent infectious disease transfer as a result of contact with contaminated swimming pool.