In this experiment, six accessions of Satureja bakhtiarica and S. spicigera from Yazd, Esfehan and Gilan provinces, Iran, were collected and cultivated in the form of a randomized complete block design with three replications. At flowering stage, the following traits were measured: canopy diameter, plant height, total number of branches, number of flowering branches, fresh and dry yield, and essential oil percentage and yield. At the flowering stage, plants were harvested and dried under shadow, and the essential oil was produced by hydrodistillation using a Clevenger in 2 hours. Analysis of variance indicated the two species varied in their shoot dry yield. There were significant differences in plant height (P≤0.01) and number of main branches, canopy diameter, essential oil percentage and yield (P≤0.05) of the different accessions. Mean comparison of the species showed that the accession Yazd 18 had the highest plant height (45.78 cm). Regarding the number of main branches, Yazd 18, Yazd 19, Gilan 32 and Gilan 35 were in a same group and Gilan 29 and Esfehan 26 were in another group. Shoot dry yield was the highest (903 kg/ha) in Gilan 35. The essential oil yield was the highest in Gilan 29, Gilan 32 and Gilan 35 (8.30, 8.57 at 8.02 kg/ha, respectively). Results also indicated that dry yield was significantly correlated to essential oil yield.