Anxiety is one of the most important usual disorders in human communities which cause many other diseases. Considering numerous side effects of tranquilizers and anti-anxiety chemical drugs, people tend to use herbal drugs to treat anxiety disorders increasingly. Current study was carried out to compare the effects of hop’s hydro alcoholic extract and diazepam on reducing the anxiety of laboratory mice. Sixty female mice from the weight range of 25-30 gram were divided into six groups (control, anxiety, diazepam and 50, 100 and 200 mg extract doses). Anxiety was enforced using dark box and measured using plus elevated maze. Elapsed time and the number of presence in open arms for five minutes were recorded as anxiety indices. Obtained data were analyzed using variance analysis tests. Results showed that extract in all doses increased average open arm time and movement activity in proportion to anxiety group. According to results, hop extract in all dosed could reduce the anxiety and acted like diazepam. Therefore, it is recommended as a replacement for diazepam.
