Today, as the main sources of food are limited, the importance of efficient use of different sources and also reduction of wastes seems necessary. Button mushroom (Agaricus bisporus) is a rich source of food and its stipe is usually considered as wastes. The purpose of this study is to determine some chemical compounds and nutrient materials of stipe in comparison with cap of button mushroom in three different flushes. Mushrooms were harvested in three different flushes and some chemical characteristics such as moisture, protein, carbohydrate, fat, fiber and ash were analyzed for cap and stipe separately. All tests were performed in three replicates. From the first to the third flush, the amount of moisture, carbohydrate and fat decreased significantly and the protein and fiber content showed significant increase in cap. The behavior of moisture and fat content in different flushes for cap and stipe was similar. Moisture and fat content of stipe, same as cap decreased significantly (p<0.05) from first flush to the third flush. The fiber content of stipe was significantly (p<0.05) higher than cap and the average of fiber content in three different flushes reported as 27.11 for cap and 38.51 for stipe. According to our results, both parts of mushroom (cap and stipe) are valuable sources of nutrition and using both parts in diet might be effective in human health. Also the stip part of button mushroom can be applied as a valuable food source for quantitative and qualitative provision of some human needs, and help the economy of the country by applying agriculture.