The present investigation is based on a comparative standardization and physicochemical analysis of the dried fruits of Tribulus terrestris L. collected from three different geographical locations of South India viz., Ballery (Karnataka), Nakrekal (Telangana), Katumnar koil (Tamilnadu) in June and December seasons. The various standardization parameters included determination of foreign matter, loss on drying, ash values, extractive values, fluorescence analysis and preliminary phytochemical screening was performed. The preliminary identification was done by using macroscopical characters and final identity was confirmed by botanist. The identification was also carried out with fluorescence analysis of the powdered fruits treating with various chemical reagents. The result from present study deals with the major variations observed in physicochemical characters in Tribulus terrestris due to change in season and region. The study proved that as the geographical and seasonal variation has direct influence with the variation in physicochemical constituents of the plants.
